The Genetic Engineering Core Facility has been developed thanks to the funding of the Foundation for Polish Science - Team Tech Core Facility Plus project, curently working as a unit of Nencki Animal House. The facility provides services in the transgenic field, offering the generation, genotyping and cryopreservation of genetically modified animals, as well as viral vectors production (LV and AAV). Additionally, we perform the restoration of animal SPF status by embryo transfer, rederivation from cryopreserved stocks, and IVF procedures. Behavioral analysis of generated transgenic lines is available in the Laboratory of Behavioral Methods at the Nencki Institute.

Our service is dedicated to scientific and R&D institutions.

A List Of Transgenic Animals Produced In Our Facility: 

• Tet-GFP transgenic rat with tetracycline control of gene expression (Konopka 2009)

• cFos-PSD95-Venus-Arc - transgenic rat (Knapska et al., 2012)

• Syn-MMP9 transgenic rat model with neurospecific overexpression of MMP-9  (Wilczynski et al., 2008)

• Syn-Flag-ICER transgenic rat model with neurospecific overexpression of ICER (Inducible cAMP Early Repressor)

• Syn-TDP43 transgenic rat model with neurospecific overexpression of TDP-43 (LV mediated transgenesis) (Koza et al., 2019)

• Thy1-Tweety transgenic rat model with neurospecific overexpression of Tweety gene (in cooperation with prof. K. Łukasiuk, Nencki Institute, NCN OPUS grant)

• Tph-DREADD-mCherry transgenic rat model with serotoninergic specific overexpression of activating DREADD (in cooperation with prof. Urszula Sławinska, Nencki Institute, NCN OPUS grant)

• 3 transgenic mice lines with neurospecific overexpression of Thy1-ORAI1,Thy-STIM-1, Thy1-STIM-2  used as Alzheimer disease models (Majewski et al., 2017, Maciag et al., 2019, Majewski et al., 2019, Majewski et al., 2020) (for prof. Jacek Kuźnicki, IIMCB PAS Warsaw)

• 3 transgenic mice models for opioid system studies overexpression of Drdla BAC, DAT-OPRK-1 KD-BAC and Dbh-BAC (for dr Jan Rodriguez-Parkitna, Pharmacology Institute, PAS Kraków)

• NWC transgenic mice model for immunological system development studies (in cooperation with Hirszfeld Immunology and Experimental Therapy Institute PAS)

• MMP9-BAC-EGFP - transgenic mice coding enhanced fluorescent protein EGFP gene under the extracellular matrix metalloproteinase 9 (MMP-9) promoter