Cryopreservation

Cryopreservation is time and cost saving method enabling preservation of valuable transgenic lines that are not currently in use.

Freezing of sperm or embryos is always recommended, as they constitute as a ‘back-up’ in case of disease outbreak, fertility problems, spontaneous phenotype loss, etc. A complete cycle of cryopreserving a strain and reconstituting it from frozen material involves the same biological steps whether one uses embryos or sperm. In both cases, the production of fertilized embryos is the most expensive step. With embryo cryo, the fertilization step takes place before freezing, whereas with sperm cryo it is done after thawing.

There are 2 possible embryo cryopreservation options depending on mice genotype:

Option 1 - Cryopreservation of heterozygous animals. Investigator provides 5-10 stud males (3-6 months old). The Facility provides  ~30 donor females (C57Bl6/J or FVB), which allowing to collect ~ 300 embryos/line at morula/blastocyst stage of development. 

Option 2 - Cryopreservation of homozygous animals. Investigator provides 5-10 stud males (3-6 months old) and ~20 donor females (5-8 weeks old), enabling to receive ~200 morulas/blastocysts.

Embryo Freezing Order Form

For sperm cryopreservation we will freeze the sperm isolated from 3-5 practiced males (3-6 month of age) per line 

Sperm Cryopreservation Order Form

For more information and ordering details please contact us: 

j.przybys@nencki.edu.pl

a.klejman@nencki.edu.pl